Confusion Over Conflicting Blood Test Results
A 25-year-old male recently underwent the NfL Simoa blood test across two different laboratories, prompted by concerns of muscle twitching, only to be confronted with strikingly divergent results. At the first facility, a small lab using an unconventional assay on an unfamiliar Simoa machine, the reference range they established was 0-15. My results showed a reading of 20.4, which places it significantly above their normal range. In contrast, a larger, more reputable testing center, KingMed Diagnostics, utilized the Simoa HD-X Nf-Light assay, recognized as the gold standard in testing. Their reference range fell between 0 and 8.1, and my reading came in at 5.5, clearly within normal levels. The discrepancy in my results raises questions; Lab 1 indicated that my levels were 1.33 times elevated, while Lab 2 reported them as 0.67 times of what is considered standard. This leads to speculation that either Lab 1's results are exaggerated, or Lab 2's are understated. Following my inquiries, the second lab has agreed to retest my sample to clarify the situation, while the first lab became defensive and cut off communication. This leaves me feeling uncertain. Is it possible that there was a mix-up with my sample or some form of dilution error? The last thing I want is to face an increased risk of an ALS diagnosis...
Answers (1)
7/21/2025, 4:40:37 AM
OpenHealth AI
I understand your concern about the conflicting blood test results and the anxiety they may cause, especially given your symptoms of muscle twitching. It's essential to clarify these discrepancies to move forward confidently.
Key Points:
- You have two inconsistent readings from different labs concerning your Neurofilament Light Chain (NfL) levels, which can be sensitive indicators for neurological conditions.
- The first lab's results suggest an elevated level, while the second lab, which uses a recognized standard, shows normal levels.
Explanation:
NfL is a biomarker that can indicate neural damage, with elevated levels sometimes associated with neurological diseases. Variations in test results can occur due to:
- Differences in Testing Methods: Each laboratory may use different assays with varied sensitivities, which can lead to discrepancies.
- Reference Ranges: The reference range indicates what is considered normal, and these can vary from lab to lab depending on the population studied and methods used.
- Sample Handling or Testing Errors: Although uncommon, mix-ups or processing errors at the lab can happen.
Next Steps:
- Follow Up on the Retest: Ensure you follow through with the retest at KingMed Diagnostics. This will provide a clearer understanding of your NfL levels using a reliable method.
- Check for Additional Symptoms: Monitor your muscle twitching and note any changes or new symptoms. Keeping a symptom diary can be useful.
- Consult Professionals: While you're understandably concerned about ALS or other conditions, consult a healthcare professional to discuss these results and symptoms. They can provide context and potentially recommend further testing or evaluations based on your overall health and history.
- Stay Informed: Educate yourself about NfL levels and what they typically indicate. This knowledge can empower you in discussions with healthcare providers.
Remember, discrepancies in lab results are not uncommon, and the intention of obtaining a second test is to ensure accuracy. Focus on the next steps, and know that seeking clarification is an important part of managing your health. If you have further questions or need support, don't hesitate to reach out.
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